Protein_Domain
FRB

Part:BBa_J18926:Design

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)


FRB(T2098L)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 225
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • aa translation from human mTOR,
  • mutation T2089L (verified against pC4-RHE from Ariad)
  • GeneArt codon optimization for E. coli
  • gene synthesis


Source

human mTOR (aa sequence)

gene synthesis

References